بهینه‌سازی، مشخصه‌یابی و فعالیت آنتی‌باکتریایی نانوذرات طلای بیوسنتز شده با استفاده از عصاره آبی گیاه Sambucus ebulus L.

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس‌ارشد، گروه شیمی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران

2 دانشیار، عضو هیأت علمی گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران

3 استاد، عضو هیأت‌علمی گروه شیمی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران

4 مربی، عضو هیأت‌علمی گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران

5 استادیار، عضو هیأت‌علمی گروه تولیدات گیاهی، دانشکده کشاورزی، مجتمع آموزش عالی سراوان، سراوان، ایران

چکیده

گیاهان به‌عنوان منابع پایدار و دسترس در تهیه نانوذرات زیست سازگار در سال‌های اخیر مورد توجه زیادی قرار گرفته‌اند. هدف از مطالعه حاضر بهینه‌سازی و مشخصه‌یابی و بیوسنتز نانو ذرات طلا با استفاده از عصاره آبی برگ گیاه آقطی (Sambucus ebulus L.) می‌باشد. پس از آماده کردن عصاره، 2 میلی‌لیتر از آن به 4 میلی‌لیتر از نمک طلا (HAuCl4.3H2O) با غلظت 1 میلی‌مولار اضافه شد که عصاره، یون‌های طلا (III) را به اتم‌های طلا در ابعاد نانومتریک کاهش داده و بلافاصله رنگ محلول به رنگ بنفش تغییر داد. به‌منظور دستیابی به نانوذرات طلا با شکل و اندازه یکنواخت، پارامترهای موثر بر سنتز، نظیر pH محیط واکنش، حجم عصاره، غلظت نمک طلا، دما و زمان واکنش مورد مطالعه قرار گرفت و تمامی پارامتر‌ها با استفاده از اسپکتروفتومتری فرابنفش- مرئی بهینه شدند. برای مشخصه یابی نانوذرات از تصویر میکروسکوپ الکترونی عبوری (TEM) و پراش پرتو ایکس (XRD) استفاده شد. در نهایت خواص آنتی باکتریایی نانوذرات بر روی 4 گونه باکتری بیماری‌زا استافیلوکوکوس اورئوس، باسیلوس سوبتیلیس، اشرشیا کلی و سالمونلا اینتریتیدیس) با روش دیسک دیفیوژن مورد بررسی قرار گرفت و به‌صورت قطر‌هاله عدم رشد گزارش شد. نتایج نشان دادند که نانو ذرات طلا کروی بوده و علاوه بر اینکه حداکثر جذب را در 534 نانومتر دارند، با میانگین اندازه بین 17-11 نانومتر از فعالیت آنتی‌باکتریایی نسبتاً خوبی نیز علیه باکتری‌های برخوردارند.

کلیدواژه‌ها

موضوعات

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