بهینه‌سازی، مشخصه‌یابی و فعالیت آنتی‌باکتریایی نانوذرات طلای بیوسنتز شده با استفاده از عصاره آبی گیاه Sambucus ebulus L.

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس‌ارشد، گروه شیمی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران

2 دانشیار، عضو هیأت علمی گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران

3 استاد، عضو هیأت‌علمی گروه شیمی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران

4 مربی، عضو هیأت‌علمی گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه سیستان و بلوچستان، زاهدان، ایران

5 استادیار، عضو هیأت‌علمی گروه تولیدات گیاهی، دانشکده کشاورزی، مجتمع آموزش عالی سراوان، سراوان، ایران

چکیده

گیاهان به‌عنوان منابع پایدار و دسترس در تهیه نانوذرات زیست سازگار در سال‌های اخیر مورد توجه زیادی قرار گرفته‌اند. هدف از مطالعه حاضر بهینه‌سازی و مشخصه‌یابی و بیوسنتز نانو ذرات طلا با استفاده از عصاره آبی برگ گیاه آقطی (Sambucus ebulus L.) می‌باشد. پس از آماده کردن عصاره، 2 میلی‌لیتر از آن به 4 میلی‌لیتر از نمک طلا (HAuCl4.3H2O) با غلظت 1 میلی‌مولار اضافه شد که عصاره، یون‌های طلا (III) را به اتم‌های طلا در ابعاد نانومتریک کاهش داده و بلافاصله رنگ محلول به رنگ بنفش تغییر داد. به‌منظور دستیابی به نانوذرات طلا با شکل و اندازه یکنواخت، پارامترهای موثر بر سنتز، نظیر pH محیط واکنش، حجم عصاره، غلظت نمک طلا، دما و زمان واکنش مورد مطالعه قرار گرفت و تمامی پارامتر‌ها با استفاده از اسپکتروفتومتری فرابنفش- مرئی بهینه شدند. برای مشخصه یابی نانوذرات از تصویر میکروسکوپ الکترونی عبوری (TEM) و پراش پرتو ایکس (XRD) استفاده شد. در نهایت خواص آنتی باکتریایی نانوذرات بر روی 4 گونه باکتری بیماری‌زا استافیلوکوکوس اورئوس، باسیلوس سوبتیلیس، اشرشیا کلی و سالمونلا اینتریتیدیس) با روش دیسک دیفیوژن مورد بررسی قرار گرفت و به‌صورت قطر‌هاله عدم رشد گزارش شد. نتایج نشان دادند که نانو ذرات طلا کروی بوده و علاوه بر اینکه حداکثر جذب را در 534 نانومتر دارند، با میانگین اندازه بین 17-11 نانومتر از فعالیت آنتی‌باکتریایی نسبتاً خوبی نیز علیه باکتری‌های برخوردارند.

کلیدواژه‌ها

موضوعات


  1. Abdollahi, H., Javadi, H., Zand monfared, M.R. 2014. Synthesis of gold nanoparticles and study of their antimicrobial effects study on Helicobacter pylori. Journal of Qom University of Medical Sciences, 8(2): 44-50. (In Persian)
  2. Amanda, S., Mohammad, F., John, J., Schlager, D., Syed, A. 2010. Metal-based nanoparticles and their toxicity assessment. Journal of Nano medicine and Nanotechnology, 2: 544-568.
  3. Armendariz, V., Herrera, I., Peralta-Videa, J. R., Jose-Yacaman, M., Troiani, H., Santiago, P., Gardea-Torresdey J.L. 2004. Size controlled gold nanoparticle formation by Avena sativa biomass: use of plants in nano biotechnology. Journal of Nanoparticle Research, 6: 377–382.
  4. Asghari, J.H., Gorganli Doji, T., Ghaemi, A. 2014. Phytochemical and antimicrobial investigation of essencial oil of Ferula gummosa Boiss. in Meighan region Semnan province. Eco-phytochemical Journal of Medicinal Plants, 1(5): 28-35 (In Persian)
  5. Ashiri, S., Safari, J. 2013. Synthesis of gold and silver nanoparticles in plant substrates and their application. Nanotechnology, 1(186): 12-15. (In Persian)
  6. Basiri, Sh. 2011. Investigation of the effect of temperature and air velocity in the dryer on the amount and quality of essential oil of Thymus. Innovation of Science and Food Technology Journal, 3(4): 73-82. (In Persian)
  7. Cho, K. J., Park, T., Osaka, S. 2005. The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochimica Acta, 51: 956-960.
  8. Dubeya, Sh. P., Lahtinen, M., Sillanpaaa, M. 2010. Tansy fruit mediated greener synthesis of silver and gold nanoparticles. Process Biochemistry, 45: 1065–1071.
  9. Dwivedi, A.D., Gopal, K. 2010. Biosynthesis of silver and gold nanoparticles using Chenopodium album leaf extract. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 369: 27–33.
  10. Fayaz, A.M., Balaji, K., Girilal, M., Yadav, R., Tchc, M., Kalaichelvan, P.T., Venketesan R. 2010.  Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. Nanomedicine, 6: 103-109.
  11. Foroghirad, S., Khatibzadeh, M. 2015. Green synthesis of silver nanoparticles used in conductive inks using sonochemical method. Iranian Journal of Chemistry and Chemical Engineering, 1(34): 1-9. (In Persian)
  12. Gardea-Torredey, J. L., Parsons, J. G., Gornez, E., Peralta-Videa, J., Troiani, H. E., Santiago P. 2002. Formation and growth of Au nanoparticles inside live alfala plants. Nano letters, 2: 397-401.
  13. Gardea-Torresdey, J. L., Tiemann, K., J, Gamez, G., Dokken, K., Tehuacanero, S., Jose-Yacaman, M. 1999. Gold nanoparticles obtained by bio-precipitation from Gold (III) solutions. Journal of Nanoparticle Research, 1(3): 397-404.
  14. Govindaraju, K., Tamilselvan, S., Kiruthiga, V., Singaravelu, G. 2010. Biogenic silver nanopar ticles by Solanum torvum and their promising antimicrobial activity. Journal of Bio pesticides, 3(1): 394–399.
  15. Grace, A.N., Pandian, K. 2007. Antibacterial efficacy of aminoglycosidic antibiotics protected gold nanoparticles: A brief study, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 297(1-3): 63-70.
  16. Herrera, I., Gardea-Torresdey, J.L., Tiemann, K.J., Peralta-Videa, R., Armendariz, V., Parsons, J.G. 2003. Binding of silver (I) ions by alfalfa biomass (Medicago sativa). Batch, time, temperature, and ionic strength studies. Journal of Hazardous Substance Research, 4: 1-16
  17. Inbakandan, D., Venkatesan, R., Khan, S.A. 2010. Biosynthesis of gold nanoparticles utilizing marine sponge Acanthella elongata. Colloids and Surfaces: B, 81: 634–639.
  18. Jones, G.L., Muller, C.T., O’Reilly, M., Stickler, D.J. 2006. Effect of triclosan on the development of bacterial biofilms by urinary tract pathogens on urinary catheters. Journal of Antimicrobial Chemotherapy, 57: 266-272.
  19. Kalishwaralal, K., Deepak, V., Ram Kumar Pandian, S., Kottaisamy, M., Barathmanikanth, S., Kartikeyan, B., Gurunathan, S. 2010. Biosynthesis of gold and silver nanoparticles using Brevibacterium casei. Colloids Surfaces Bio interfaces, 77(2): 257-262.
  20. Kamali, M., Ghorashi, S.A.A., Asadollahi, M.A. 2012. Controllable synthesis of silver nanoparticles using citrate as complexing agent: characterization of nanopartciles and effect of  pH on size and crystallinity. Iranian Journal of Chemistry and Chemical Engineering, 31(4): 21-28.
  21. Kasture, M.B., Patel, P., Prabhune, A.A., Ramana, C.V., Kulkarni, A.A., Prasad, B.L.V. 2008. Synthesis of silver nanoparticles by sophorolipids: effect of temperature and sophorolipid structure on the size of particles. Journal of Chemical Sciences, 120 (6): 515–520.
  22. Kaviya, S., Santhanalakshmi, J., Viswanathan, B. 2011. Green Synthesis of Silver Nanoparticles Using Polyalthia longifolia Leaf Extract along with D-Sorbitol: Study of Antibacterial Activity. Journal of Nanotechnology, 2011: 1-5.
  23. Lin, D.H., Xing, B.S. 2007.  Phytotoxicity of nanoparticles: Inhibition of seed germination and root growth. Environmental Pollution, 150: 243-250.
  24. Louis, C., Pluchery, O. 2012. Gold nanoparticles for physics, chemistry and biology, imperial college press, London, United Kingdom, 395.
  25. Marshal, A.T., Haverkamp, R.G., Davies, C.E., Parsons, J.G., Gardea-Torresdey, J.L., Agterveld, D.V. 2007. Accumulation of gold nanoparticles in Brassica Juncea. International Journal of Phytoremediation, 9: 197-206.
  26. Mironov, I.V., Makotchenko, E.V. 2009. The hydrolysis of AuCl−4 and the stability of aqua chlorohydroxo complexes of gold (III) in aqueous solution. Journal of Solution Chemistry, 38(6): 725-737.
  27. Moadi, T., Ghahramanzadeh, R., Yosofi, M., Mohammadi, F. 2014. Synthesis of silver nanoparticles using four species plant and investigation of their antimicrobial activity. Iranian Journal of Chemistry and Chemical Engineering, 33(4): 1-9. (In Persian)
  28. Mock, J.J., Barbic, M., Smith, D.R., Schultz, D.A., Schultz, S. 2002. Shape effects in plasmon resonance of individual colloidal silver nanoparticles. The Journal of Chemical Physics, 116: 6755-6759.
  29. Mukkerjee, A., Ahmad, A., Mandal, D., Senapti, S., Sainkar, S.R., Khan, M.I., Parishcha, R., Ajaykumar, P.V., Alam, M., Kumar, R., Sastry, M. 2001. Fungus-mediated synthesis of silver nanoparticles and their immobilization in mycelial matrix: a novel biological approach to nanoparticles synthesis. Nano letters, 1: 515-519.
  30. Narayanan, K.B., Sakthivel, N. 2011. Green synthesis of biogenic metal nanoparticles by terrestrial and aquatic phototrophic and heterotrophic eukaryotes and biocompatible agents. Journal of Advances in Colloid and Interface Science, 169: 59-79.
  31. Nejati, K., Davari, S. 2009. The effect of size of the nanoparticles on optical properties of plasmon line width. Nanotechnology,5(142):44-46.(In Persian)
  32. Philip, D. 2010. Green synthesis of gold and silver nanoparticles using Hibiscus Rosasinensis. Physica E, 42: 1417–1424.
  33. Ramzani, F., Kazemi, B., Jebali, A. 2013. Biological synthesis of silver nanoparticles. New cellular and Molecular Biotechnology Journal, 3(9):107-111. (In Persian)
  34. Rai, A., Singh, A., Ahmad, A., Sastry, M. 2006. Role of halide ions and temperature on the morphology of biologically synthesized gold nanotriangles. Langmuir, 22 (2): 736–741.
  35. Rai, M., Yadav, A., Gade, A. 2009. Silver nanoparticles as a new generation of antimicrobials. Biotechnology Advances, 27: 76-83.
  36. Ruparelia, J.P., Chatterjee, A.K., Duttagupta, S.P., Mukherji, S. 2011. Strain specificity in antimicrobial activity of silver and copper nanoparticles. Free Radical Biology and Medicine, 363: 481- 489.
  37. Shah Mirzaee, H., Pazoki, M. 2007. A review on production of nanoparticles using microorganisms. Nanotechnology, 6 (119): 349-355. (In Persian)
  38. Shankar, S. S., Ahmad, A., Pasricha, R., Sastry, S. 2003. Bio reduction of chloroaurate ions by Geranium leaves and its endophytic fungus yields gold nanoparticles of different shapes. Journal of Materials Chemistry, 13: 1822-1826.
  39. Shanker, S.S., Rai, A., Ankamwar, B., Singh, A., Ahmad, A., Sastry, M. 2004. Biological synthesis of triangular gold nanoprisms. Nature Material, 3: 482-488.
  40. Shenya, D.S., Mathewa, J., Philip, D. 2011. Phytosynthesis of Au, Ag and Au–Ag bimetallic nanoparticles using aqueous extract and dried leaf of Anacardium occidentale. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 79: 254–262.
  41. Song, J.Y., Kim, B.S. 2009. Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess and Biosystems Engineering, 32 (1): 79–84.
  42. Tajarrodi, A., Kiazadeh, A., 2007. The Comparison of the methods to synthesis of silver nanoparticles. Nanotechnology, 6(117): 217-222. (In Persian)
  43. Thakkar, K.N., Mhatre, S.S., Parikh, R.Y. 2010. Biological synthesis of metallic nanoparticles. Nanomedicine, 6: 257-262.
  44. Tiwari, D.K., Behari, J., Sen, P. 2008. Time and dose dependent antimicrobial potential of Ag nanoparticles synthesized by top-down approach. Current Science, 95: 647–655.
  45. Vinoda, V.T.P., Saravanan, P., Sreedharc, B., Keerthi Devic, D., Sashidhard, R.B. 2011. A facile synthesis and characterization of Ag, Au and Pt nanoparticles using a natural hydrocolloid gum kondagogu (Cochlospermum gossypium). Colloids and Surfaces B: Bio interfaces, 83: 291–298.
  46. Waghmar, S.S., Deshmukh, A.M., Sadowski, Z. 2014. Biosynthesis, optimization, purification and characterization of gold nanoparticles. African journal of microbiology research, 8(2): 138-146.
  47. Wiley, B.J., Im, S.H., Li, Z.Y., McLellan, J., Siekkinen, A., Xia, Y. 2006. Maneuvering the surface plasmon resonance of silver nanostructures through shape-controlled synthesis. Journal of Physical Chemistry B, 110(32): 15666–15675.
  48. Yoon, K., Byeon, J.H., Park, J., Hwang, J. 2007. Susceptibility constant of Escherichia coli and Bacillus subtilis to silver and copper nanoparticles. Science of Total Environment, 373: 572-575.
  49. Zakeri, M., Fasihi, J. 2011. Synthesis of gold nanoparticles using biomass of wheat and investigation of effective parameters. Iranian Journal of Chemistry and Chemical Engineering, 30(2): 35-41. (In Persian).