بررسی فیتوشیمیایی، آنتی‌اکسیدانی و ضدالتهابی استرول‌ها و اسیدهای چرب دو گونه دارویی( Eryngium caucasicum Trautv) و (caucalis platycarpos L) در استان گیلان

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه باغبانی شاخه گیاهان دارویی، دانشگاه پردیس گیلان، رشت، ایران

2 استاد، گروه علوم باغبانی، دانشگاه گیلان، رشت، ایران

3 فارماکوگنوزی/ دانشکده داروسازی/ دانشگاه علوم پزشکی شیراز

چکیده

التهاب واختلالات وابسته به فرآیند اکسیداسیون زمینه ساز بسیاری از بیماری‌های تهدید کننده سلامت عمومی را میباشند. در این تحقیق به بررسی و شناسایی محتوای استرولی و اسیدهای چرب موجود در دو گونه گیاه E.caucasicum و C.platycarpos پرداخته شده است. دو گونه گیاهی یاد شده متعلق به تیره چتریان(Apiaceae) بوده که نقش قابل توجهی در فرهنگ تغذیه‌ ای منطقه خزری دارامیباشند. علاوه بر بررسی فیتوشیمیایی این دو گونه‚ پتانسیل‌های ضد التهابی و آنتی‌اکسیدانی آنها نیز به منظور آماده سازی مکمل‌های غذایی مورد بررسی قرار گرفت. ارزیابی اسیدهای چرب و فیتواسترول‌های این گیاهان مذکوربا روش کروما توگرافی گازی- طیف سنجی جرمی chromatography-Mass spectroscopy مورد ارزیابی قرار گرفت و برای شناسایی ساختار ترکیبات شناسایی شده از مقایسه کروماتوگرام‌های حاصل با اجزای استاندارد، استفاده شد. برای بررسی اثرات آنتی‌اکسیدانی از متدهای زیر بهره گرفته شد: 1)) 1, 1-diphenyl-1-picrylhydrazyl (DPPH) 2) phosphomolybdenum, 2 2’-azino-bis (3-ethylbenzothiazoline)-6-sulphonic acid radical cation (ABTS) )3) cupric ion reducing activity (CUPRAC) )4) Ferric reducing antioxidant power (FRAP) و )5) metal chelating activity on ferrous ions. همچنین برای ارزیابی فعالیت ضد التهابی اسیدهای چرب و فیتواسترول‌های موجود در فراکسیون‌های دو گونه مذکور از روش human red blood cell (HRBC) membrane stabilization method استفاده شد. مقدار کلی روغن این گیاهان به ترتیب 18.21% و 7.39 % بود که درصد روغن موجود در عصاره گیاه E.caucasicum به طور قابل توجهی از C.platycarpos بیشترمیباشد.

کلیدواژه‌ها

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