بررسی اثر آلودگی‌های صنعتی بر تجمع آرسنیک در خاک و برخی واکنش‌های فیزیولوژیکی و بیوشیمیایی گیاه دارویی .Malva neglecta L

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشکده کشاورزی و منابع طبیعی، گروه کشاورزی، دانشگاه آزاد اسلامی واحد گرگان، گرگان، ایران

2 دانش آموخنه کارشناسی ارشد زیست گیاهی دانشگاه آزاد اسلامی واحد گرگان

چکیده

آلودگی‌های زیست محیطی، از جمله فلزات سنگین یکی از معیارهای کنترل کیفیت گیاهان دارویی و محصولات فرآوری شده‌ی آنها می‌باشند. به منظور بررسی اثر آلودگی‌های صنعتی بر برخی واکنش‌های فیزیولوژیکی و بیوشیمیایی گیاه پنیرک (Malva neglecta L.)، آزمایشی به صورت فاکتوریل در قالب طرح بلوک کامل تصادفی با سه تکرار در اراضی کشاورزی اطراف شهرک صنعتی بندر گز در استان گلستان در بهار 1392 اجرا گردید. فاکتور اول شامل فاصله از منبع آلودگی در چهار سطح (شاهد، 100، 200و 300 متر) و فاکتور دوم نوع اندام گیاه (هوایی و ریشه) بود. کرت شاهد نیز در همان منطقه در فاصله 1000 متری از منبع آلودگی در نظر گرفته شد. در این آزمایش مقدار آنتوسیانین، قند محلول کل، فلاونوئید، پروتئین، پرولین، آنزیم پراکسیداز، کاتالاز، گلیسین بتائین و آرسنیک و کادمیوم خاک و گیاه اندازه‌گیری شدند. نتایج نشان داد بیشترین میزان آنتوسیانین (37/6 میکرومول در گرم وزن تر اندام هوایی)، ، قند محلول (04/42 میلی‌گرم در گرم وزن خشک اندام هوایی)، پرولین (05/15 میکرومول در گرم وزن خشک اندام هوایی) و گلیسین بتائین (99/46 میکروگرم در گرم وزن خشک اندام هوایی) در تیمار 200 متر فاصله از منبع آلودگی به دست آمد. بیشترین میزان پروتئین (099/0 گرم در کیلوگرم وزن خشک)، فلاونوئید (305/1 میلی‌گرم در گرم وزن خشک اندام هوایی)، آرسنیک (37/515 میکروگرم در کیلوگرم اندام هوایی) و فعالیت آنزیم‌های کاتالاز و پراکسیداز (به ترتیب 84/8 و 62/25 میکرومول آب اکسیژنه بر دقیقه ) مربوط به تیمار 100 متر فاصله از منبع آلودگی بود.

کلیدواژه‌ها

موضوعات


References

  1. Assareh, M. H. and Shariat, A., 2009. Study of salt tolerance in germination and vegetative growth in four species of Eucalyptus. Journal of Agricultural Sciences and Natural Resources of Gorgan, 15 (6): 145-157.
  2. Agarwal, S. and Pandey, V., 2004. Antioxidant enzyme responses to NaCl stress in Cassiaangustifolia. Plant Biology, 48, 555-560.
  3. Alamgir., A.N.M. and Akhter, S., 2010. Effects of aluminum on some biochemical characteristics of wheat (Triticum aestivum L.). Bangladesh Journal of Botany, 39, 9-14.
  4. Arabi, Z., Homaee, M., Asadi, M. A. and Asadi Kapoul, S., 2017. Cadmium removal from Cd-contaminated soils using some natural and synthetic chelates for enhancing phytoextraction.Chemistry and Ecology, 33(5), 389-402.
  5. Ashraf, M. and Foolad, M.R., 2007. Roles of glycinebetaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59, 206–216.
  6. Bertrand M. and Schoefs B., 1999. Photosyntetic pigment metabolism in plants during stress, In: Handbook of plant and crop stress (ed. Pessarakli, M.), Marcel Dekker, New York. 527-543.
  7. Boroomand jazi., Sh., Lari Yazdi, H. and Ranjbar, M., 2011. Effect of salicylic acid on photosynthetic pigments, sugar content and antioxidant enzymes in rapeseed under lead stress, Plant Biology, 3 (9), 39-52.
  8. Chance, B. and Maehly, A.C., 1955. Assay of catalases and peroxidases. Methods in Enzymologist, 11, 764-755.
  9. Chang, C.C., Yang, M.H., Wen, H.M.and Chern, J.C., 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10, 178-182.
  10. Dai, L.P., Xiong, Z.T., Huang,Y. and Li, M. J., 2006. Cadmium‐induced changes in pigments, total phenolics, and phenylalanine ammonia‐lyase activity in fronds of Azolla imbricata. Environmental toxicology, 21(5), 505-512.
  11. Diaz, J., Bernal, A.,Pomar, F. and Merino, F., 2001. Induction of shikimate dehydrogenase and peroxidase in pepper (Capsicum annum L.) seedlings in response to copper stress and its relation to lignification. Plant Science, 161, 179p.
  12. Dubey, R.S. and Singh, A.K., 1999. Salinity induces accumulation of soluble sugars and alters the activity of sugar metabolizing enzymes in rice plants. Biol. Plantarum, 42, 233-239.
  13. Ewaise, E.A., 1997. Effects of cadmium, nickel and lead on growth, chlorophyll content and proteins of weed. Biologica Plantarum, 39(3), 403-410.
  14. Ghelich, s., Zarinkamar, F. and Niknam,V., 2015.Investigating the amount of lead accumulation and its effect on peroxidase activity, phenolic and flavonoids content in germination stage in alfalfa (Medicago sativa L.).  Journal of Plant Research, 28 (1): 164-174.
  15. Ghosh, M., and Singh, S.P., 2005. A comparative study of cadmium phytoextraction byaccumulator and weed species. Environmental Pollution, 133(2): 365-371.
  16. Girija, C., Smith, B. N. and Swamy, P.M., 2002. Interactive effects of sodium chloride and calcium chloride on the accumulation of proline and glycinebetaine in peanut (Arachis hypogaea L.). Journal ofEnvironmental and Experimental Botany. 47 (1): 1-10.
  17. Gupta, P.K., 2000. Soil, plant, water and fertilizer analysis. New Delhi: Agrobios.
  18. Gupta, A.K., Verma, S.K., Khan, K. and Verma, R.K., 2013. Phytoremediation using aromatic plants: a sustainable approach for remediation of heavy metals polluted sites. Environmental Science & Technology, 47(18): 10115-10116.
  19. Jiang, M.and Zhang, J., 2001.Effect of abscisic acid on active oxygen species, antioxidative defencesystem and oxidative damage in leaves of maize seedlings. Plant Cell Physiology, 42: 1265-1273.
  20. Jiang, L.Y., and Yang, X.E.and He, Z.L., 2004. Growth response and phytoextraction of copper at different levels in soils by Elsholtzia splendens, Chemosphere, 55: 1179-1187.
  21. Janzen, H.H., 1993. Soluble salts. in: carter m.r. soil sampling and methods of analysis. crc press, Florida, 161–166.
  22. John, R., Ahmad, P., Gadgil, K. and Sharma, S., 2009. Heavy metal toxicity: effect on plant growth, biochemical parameters and metal accumulation by Brassica juncea L. international journal of plant production, 3: 1735-8043.
  23. Jung, C.H., Maeder, V., Funk, F. and Frey, B., 2003. Release of phenols from Lupinus albus L. roots exposed to Cu and their possible role in Cu detoxification. Plant and Soil, 252(2):301-312.
  24. Khavari Nezhad, R., Najafi, F., Afshar Mohammadian, M. and Falah, S.F., 2013. The Effect of Interaction of arsenic and gibberellic acid on anthocyanin and phenol in two rice cultivars.  Plant Process and Function, 14: 41-50.
  25. Khosravi, Z., Arabi, Z. and Ghorbanli, M., 2014.  Effects of some soil properties on arsenic and cadmium uptake by Silybum marianum. Journal of Soil Management and Sustainable Production, 5(3):205-218.
  26. Khudsar, T., Uzzafar, M. and Iqbal, M., 2001. Cadmium-induced changes in leaf epidermis, photosynthetic rate and pigment concentrations in Cajanus cajan. Biologia Plantarum, 44 (1): 59–64.
  27. Kochert,G.,1978.Carbohydrate determination by the phenol sulfuric acid method, In Helebust, J.A., Craig, J.S. (ed) Handbook physiological methods, Cambridge university Press, Cambridge, 96-97.
  28. Liamas, A., Ullrich, C.I. and Sanz, A., 2000. Cadmium effects on transmembrane electrical potential difference, respiration and membrane permeability of rice (Oryza sativaL.) roots. Plant and Soil, 219 (1-2): 21-28.
  29. Li, M.S., Luo, Y.P., and Su, Z.Y., 2007. Heavy metal concentrations in soils and plantaccumulation in a restored manganese mine land in Guangxi, South China. Environmental Pollution.147: 168-175.
  30. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J., 1951.Protein measurement with the Folin phenol reagent.Journal of Biological Chemistry,193 (1): 265–275.
  31. Matsouka, I., Beri, D., Chinou, I., Haralampidis, K. and Spyropoulos, C., 2011. Metals and selenium induce medicarpin accumulation and excretion from the roots of fenugreek seedlings: a potential detoxification mechanism. Plant and soil, 343 (1-2): 235-245.
  32. McLean, E. O., 1982. Soil pH and lime requirement. In: Page AL, Miller RH, Keeney DR. Methods of Soil Analysis. Part 2. Agronomy Monograph, vol. 9. American Society of Agronomy: Madison, WI, 199–223.
  33. Miteva, E.and Merakchiyska, M., 2002. Response of chloroplasts and photosynthetic mechanism of bean plants to excess arsenic in soil. Bulgarian Journal of Agricultural Science, 8: 151-156.
  34. Nelson, D.W. and Sommers, L.E., 1982. Total carbon, organic carbon, and organic matter. P. 539-579. In: Page AL (ed.) Methods of Soil Analysis. Part 2. 2nd ed. American Society of Agronomy, Madison,WI.
  35. Omidbeigi, R., 1995. Approaches to the production and processing of medicinal plants. Publishing Thought of the Day, Tehran, 50 p.
  36. Oloomi, H., 2003. The effect of cadmium on some growth indices and induction of oxidative stress in canola (Brasics napus L.), MA thesis, Kerman University.
  37. Pirooz, P.S., Manoochehri Kalantari, Kh. and Nasibi, F., 2012. Physiological investigation of sunflower plant under chromium stress: effect on growth, aggregation and induction of oxidative Stress (Helianthus annuus) in Sunflower Root, 4 (11): 73-86.
  38. Ranjbar, M., Lari Yazdi,H. and Boroumand Jazi,S.h., 2011.The effect of Salicylic acid on photosynthetic pigments, contents of sugar and antioxidant enzymes under lead stress in Brassica napus L. Journal of Plant Biology, 3 (9): 39-52.
  39. Sairam, R.K., Rao, K.V.and Srivastava, G.C., 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant science journal, 163: 1037-1046.
  40. Sakihama, Y. and Yamasaki, H., 2002. Lipid peroxidation induces by phenolic in conjunction with aluminum ions, biologia plantarum journal, 45: 249-254.
  41. Seregin, I.V. and Ivanov, V.B., 2001. Physiological aspects of cadmium and leadtoxic effects on higher plants. Russian journal of plant physiology, 48, 523-544.
  42. Sheoran, V., Sheoran, A.S. and Poonia, P., 2009. Phytomining: A review. Minerals Engineering, 22: 1007-1019.
  43. Shah, K., Kumar, R.G., Verma, S. and Dubey, R.S., 2001. Effect of cadmium on lipid peroxidation, superoxideanion generation and activities of antioxidant enzymes in growing rice seedlings. Plant Science journal, 161: 1135–1144.
  44. Shalin, A.S. and Moiseev, S.G., 2009. Optical properties of nanostructured layers on the surface of an underlying medium. Optics and Spectroscopy, 106, 916p.
  45. Tang, S., Xi, L., Zheng, J.M., and Li, H., 2003. Response to elevated CO2 of Indian mustard and sunflower growing on copper contaminated soil. Bulletin of Environmental Contamination and Toxicology. 71: 988-997.
  46. Vaseghi, S., Afuni, M., Shariatmadari, H. and Mobli, M., 2003. Effect of sewage sludge on the concentration of heavy metals in lettuce and spinach plants in different pH soils. Journal of Horticulture Science and Technology, 3: 142-125.
  47. Walkley, A. and Black, I.A., 1947. An examination of the Degtjareff method for determining organic carbon in soils: Effect of variations in digestion conditions and of inorganic soil constituents. Soil Science, 63: 251-263.
  48. Wanger, G.J., 1979. Content and vacuole/extravacuole distribution of neutral sugars, freeamino acids, and anthocyanins in protoplasts.Plant Physiology, 64: 88-93.
  49. WHO.,1998. Quality control methods for medicinal plant materials, Geneva.WHO., 2005. National policy on traditional medicine and regulations of herbalmedicines. Geneva.
  50. Wyn Jones, R.G. and Storey, R., 1981. Betaines. In: The physiology and Biochemistry of Drought Resistance in Plants. (Eds. Paleg, L.G. and Aspinall, D.). Academic Press, New York, 171-204.
  51. Yang, W.J., Rich, P.J., Axtell, J.D., Wood, K.V., Bonham, C.C., Ejeta, G., Mickelbart M.V. and Rhodes, D., 2003. Genotypic variation for glycine betaine in sorghum. Crop Science, 43: 162–169.