بررسی و مقایسه اثر روش‌های استخراج با تقطیر و سیال فوق بحرانی بر عملکرد، فعالیت آنتی‌اکسیدانی و ضدقارچی اسانس گیاه دارویی Artemisia persica Boiss.

نوع مقاله : مقاله پژوهشی

نویسندگان

1 مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان لرستان

2 باشگاه پژوهشگران جوان و نخبگان، واحد یاسوج، دانشگاه آزاد اسلامی

3 مرکز تحقیقات داروهای گیاهی رازی، دانشگاه علوم پزشکی لرستان

چکیده

دراین مطالعه سرشاخه‌های گلدار درمنه ایرانی (Artemisia persica Boiss.) در فصل تابستان سال 1396، از مزرعه تحقیقاتی نورآباد لرستان برداشت و اسانس گیاهان به روش‌های تقطیر با آب و سیال فوق بحرانی استخراج شد. مهمترین مواد موثره اسانس‌ها با استفاده از دستگاه GC/MS آنالیز گردید و عملکرد آنتی اکسیدانی و ضد قارچی آنها مورد ارزیابی قرار گرفت. با استفاده از روش براث میکرو دایلوشن اثر اسانس‌ها بر روی سویه‌های Microsporum canis ، Trichophyton rubrum، Epidermophyton floccosum و Trichophyton mentagrophytes ، سنجش شد. همچنین فعالیت آنتی‌اکسیدانی اسانس‌ها با دو آزمون مهار رادیکال های آزاد 2 و 2 - دی فنیل- 1- پیکریل هیدرازیل (DPPH) و 2 و 2- آزینو بیس 3 -اتیل بنزوتیازولین -6- سولفونیک اسید (ABTS) ارزیابی گردید. بازده اسانس در روش تقطیر با آب؛ 7/1درصد و در روش سیال فوق بحرانی بین 7/1 تا 7/5 درصد متتغییر بود. عمده‌ترین ترکیبات شناسایی شده در روش سیال فوق بحرانی عبارت بودند از؛ داوانون (29 درصد)، لاسینیاتافوران H (1/18 درصد)، آرتدوگلاسیا اکسید C (5/8 درصد) و نوروداوانه (6/7 درصد) و در روش تقطیر با آب؛ داوانون (22 درصد)، لاسینیاتافوران G (1/7 درصد) و آرتدوگلاسیا اکسید D (6 درصد)، ترکیبات غالب بودند. اسانس استخراج شده به روش تقطیر با آب دارای بیشترین اثر ضد قارچی بر سویه‌های اپیدرموفایتون فلوکوزوم و تریکوفایتون منتاگروفیتز با MFC و MIC معادل μg/mL 32، بود. همچنین اسانس استخراج شده با آب، در آزمون‌های DPPH و ABTS به ترتیب با IC50 ؛ 32/20 و 23/14 میکروگرم بر میلی‌لیتر، بیشترین قدرت مهار رادیکال های آزاد را داشت.

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