بررسی و مقایسه فیتوشیمیایی و فعالیت آنتی اکسیدانی جمعیت های مختلف بومی شیرین‌بیان ( Glycyrrhiza glabra L) در ایران

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه علوم باغبانی - دانشکده کشاورزی - دانشگاه زنجان

2 گروه باغبانی، دانشکده کشاورزی، دانشگاه زنجان. زنجان ایران

3 عضو هیئت علمی دانشگاه زنجان

4 عضو هیئت علمی پژوهشکده گیاهان و مواد اولیه دارویی، دانشگاه شهید بهشتی

5 گروه باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان ، ایران

چکیده

شیرین بیان (Glycyrrhiza glabra L.) گیاهی است از تیره پروانه‌آساها، علفی، چند ساله و ایران یکی از کشورهای صادر کننده ریشه آن محسوب می شود. این گیاه در مناطق مختلف ایران رویش دارد از این رو بررسیتنوع فیتوشیمیایی مناطق مختلف حائز اهمیت است. این تحقیق به منظور بررسی محتوای ترکیبات فنلی و فعالیت آنتی‌اکسیدانی ریشه های به قطر 2 سانتی‌متر از جمعیت‌های شیرین‌بیان در 15 استان و 30 منطقه ایران در سال 1396مهرماه برداشت و جهت آنالیز به آزمایشگاه‌های گروه باغبانی دانشگاه زنجان منتقل شد. صفات فنل کل (روش فولین سیکالتو)، فلاونوئید کل (روش آلومینیوم کلراید)، آنتوسیانین (روش اختلاف pH) و فعالیت آنتی‌اکسیدانی (روشDPPH) مورد مطالعه قرار گرفت. نتایج حاصل از تجزیه واریانس داده ها نشان داد که در بین 30 جمعیت از نظر تمامی صفات در سطح یک درصد اختلاف معنی‌داری وجود دارد. صفات فنل کل بین 05/456 تا 826 میلی‌گرم گالیک اسید بر 100 گرم، فلاونوئید کل بین 25/1019 تا 62/2291میلی‌گرم کوئرسیتن بر 100 گرم، میزان آنتوسیانین بین 89/6 تا 24/26 میلی گرم سیانیدین- 3- گلوکوزید در لیتر، فعالیت آنتی‌اکسیدانی بین 07/62 تا 14/87 درصد متنوع بود. همبستگی مثبت و معنی‌داری بین صفت فنل کل با فعالیت آنتی‌اکسیدانی در سطح یک درصد مشاهده شد، ولی با صفات فلاونوئید کل، آنتوسیانین و ارتفاع همبستگی معنی‌داری وجود نداشت. براساس نتایج حاصل از تجزیه کلاستر، 30 جمعیت شیرین‌بیان در دو گروه اصلی قرار گرفتند. ارزیابی جمعیت‌های از نظر صفات فیتوشیمیایی تنوع بالایی را نشان داد که جمعیت‌های N، KA، BA، T، E، Y، M، MR و SB را می‌توان به

کلیدواژه‌ها

موضوعات


References

  1. Bernath, J., 2001. Strategies and recent achievements in selection of medicinal and aromatic plants. In International Conference on Medicinal and Aromatic Plants. Possibilities and Limitations of Medicinal and Aromatic Plant, 576: 115-128.
  2. Hajmehdipoor, H., Aminzade, Y., Hasanlo, T., Shekarchi, M., Abedi, Z., and Piralihamedani, M., 2008. Evaluation of the quality of liqurice roots collected from different habitats of Iran. Journal Medicinal Plant, 3(27): 47-5.
  3. Kalyoncu, I.H., Akbulut, M., and Coklar, H., 2009. Antioxidant capacity, total phenolics and some chemical properties of semi-matured apricot cultivars grown in Malatya, Turkey. World Appl. Science Journal, 6: 519-523.…
  4. Nikolova, M.T., Taskova, R.M., and Peev, D.R., 2005. Exudate flavonoid aglycones of Veronica: Ecological and systematic implications. Biochemical Systematics and Ecology, 33(12): 1258–1268.
  5. Nostoc strains, originating from different climatic/geographic regions and habitats: Is their cytotoxicity environmentally dependent. Environ Toxico., 26: 20561.
  6. Omidbagi, R., 2008. Production and processing of medicinal plants. Astan Quds Razavi Publishing House. Mashhad, 47-50p.
  7. Pharsi, M., and Bagheri, A., 1988. Principles of Plant Breeding. Publishers (SID), Mashhad, 230 p. 
  8. Plants Research, 4(18): 1825-1829.
  9. Ahmadi Hosseini, S.M., Kazme Souri, M., Farhadi, N., Moghaddam, M., and Omid Baigi, R.,  2014. Changes in Glycyrrhizin Content of Iranian licorice (Glycyrrhiza glabra L.) Affected by Different Root Diameter and Ecological Conditions. Agricultural Communications, 2(4): 27-33.
  10. Amonrat, T., Soottawat, B., Wonnop, V., Eric, A., and Decker, C., 2008. The effect of antioxidants on the quality changes of cuttlefish (Sepia pharaonis) muscle during frozen storage. Journal Food Science and Technology, 41(1): 161-9.
  11. Asha, M.K., Debraj, D., Prashanth, D., Edwin, J.R., Srikanth, H.S., Muruganantham, N., Dethe, S.M., Anirban, B., Jaya, B., Deepak, M., and Agarwal, A., 2013. In vitro anti-Helicobacter pylori activity of a flavonoid rich extract of Glycyrrhiza glabra and its probable mechanisms of action.  Journal Ethnopharmacol, 145: 581–586.
  12. Becerro, M.A., and Paul, V.J., 2004. Effects of depth andlight on secondary metabolites production and cyanobacterial symbionts of the sponge Dysideagranulose. Marine Ecology Progress Series, 280: 115 -28.
  13. Bektas, T., Dimitra, D., Atalay, S., Munevver, S., and Moschos, P., 2005. Antimicrobial and antioxidant activities of the essential oil and various extract of Saliva tomentosa Miller (Lamiaceae).  Food Chemistry, 90: 333-40.
  14. Cary, D., and Wink, M., 1994. Elevation variation of quinlizidine alkaloid contents in a lupine (Lupinus argenteus) of the Rocky Mountains. Journal of Chemical Ecology, 20(4): 849 - 57.
  15. Chang, C., Yang, M., Wen, H., and Chern, J., 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3): 178-82.
  16. Chin, Y., Jung, H., Liu, Y., Su, B., Castoro, J., Keller, W., Pereira, W., and Kinghorn, D., 2007. Anti-oxidant constituents of the roots and stolons of licorice (Glycyrrhizaglabra). Agricultural and Food Chemistry, 55: 4691-7.
  17. Ebrahimzadeh, M.A., Hosseinimehr, S.J., and Hamidinia, A., 2008. Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharmacology Online, 1: 7-14.
  18. Esmaeili, H., Karami, A., Hadian, J., Saharkhiz, M.J., and Nejad Ebrahimi, S., 2019. Variation in the phytochemical contents and antioxidant activity of Glycyrrhiza glabra populations collected in Iran. Industrial Crops and Products, 137: 248-259.
  19. Farag, M.A., Porzel, A., and Wessjohann, L.A., 2015. Unequivocal glycyrrhizin isomer determination and comparative in vitro bioactivities of root extracts in four Glycyrrhiza species.  Advanced Research, 6: 99-104.  
  20. Fatima, T., Bashir, O., Gani, G., Bhat, T., and Jan, N., 2018. Nutritional and health benefits of apricots. International Journal of Unani and Integrative Medicine, 2(2): 05-09.
  21. Fattahi, M., Nazeri, V., Torras-Claveria, L., Sefidkon, F., Cusido, R.M., Zamani, Z., and Palazon, J., 2013. Identification and quantification of leaf surface flavonoids in wild-growing populations of Dracocephalum kotschyi by LC–DAD–ESI-MS. Food Chemistry, 141: 139-146.
  22. Gairola, S., Shariff, N., and Bhatt, A. 2010. Influence of climate change on production of secondary chemicals in high-altitude medicinal plants: Issues needs immediate attention. Journal of Medicinal, 4(18):1825-1829.
  23. Gairola, S., Shariff, N.M., Bhatt, A., and Kala, CP. 2010. Influence of climate change on production of secondary chemicals in high altitude medicinal plants: Issues needs immediate attention. Medicinal Plants Researcher, 4(18): 1825-9.
  24. Giusti, M., Monica, B. and Ronald, E. 2001. Characterization and measurement of Anthocyanin by UV-VIS spectroscopy. Current Protocols in Food Analytical Chemistry, F2.2.1-F2.2.13.
  25. Gobbo-Neto, L., Guaratini, T., Pessoa, C., Moraes, M., Costa-Lotufo, L., Vieira, RF., Colepicolo, P., and Lopes, NP. 2010.  Differential metabolic and biological profiles of Lychnophora ericoides from different localities in Brazilian “Campos rupestres. Brazilian Chemical Society, 21(4): 750- 9.
  26. Gohari, AR., Hajimehdipoor, H., Saeidnia, S., Ajani, Y. and Hadjiakhoondi, A. 2011. Antioxidant activity of some medicinal species using FRAP assay. Medicinal Plants, 10: 54-60.
  27. Hadian, J., Mirjalili, M.H. and Ganjpoor, N., 2011. Morphological and phytochemical characterization of natural population of Saturejakhuzestanica. Chemistry Biodiversity, 8: 1-15.
  28. Heydari, A., Hadian, J., Esmaeili, H., Kanani, M.R., Mirjalili, M.H., and Sarkhosh, A., 2019. Introduction of Thymus daenensis into cultivation: Analysis of agro - morphological, phytochemical and genetic diversity of cultivated clones. Industrial Crops and Products, 131: 14-24.
  29. Hrouzek, P., and Tomek, P., 2009. Cytotoxicology andsecondary metabolites production in terrestrial.
  30. Ibrahim, MH., Jaafar, HZE., Rahmat, A., and Abdul Rahman, Z., 2011. The relationship between phenolics and flavonoids production with total nonstructural carbohydrate and photosynthetic rate in Labisia punila benth. Under high CO2 and nitrogen fertilization. Molecules, 16: 162-74.
  31. Jafari, N., Naderi, P., and Ebrahimzadeh, M.A., 2015. Evaluation of phenolic content, total flavonoid and survey of antioxidant activity of leaves of Ficus carica and Pterocarya fraxinifolia trees using spectrophotometry and high performance liquid chromatograph methods. Iranian Journal of Plant Biology, 7(25): 1-16.
  32. Jovancevic, M., Balijagic, J., Menkovic, N., Savikin, K., Zdunic, G., Jankovic, T. and Dekic-Ivankovic, M., 2011. Analysis of Phenolic compounds in wild populations of bilberry (Vaccinium myrtillus) from Montenegro.  Journal of Medicinal Plants Research, 5(6): 910- 4.
  33. Karaman, S., Tutem, E., Bas-Kan, K.S., and Apak, R., 2010. comparison of total antioxidant capacity and phenolic composition of some apple juices with combined HPLC-CUPRAC assay. Food Chemistry, 120: 1201-9.
  34. Karampor, M., Usefi, H., and Kohpaye, N. 2015., Investigating the relationship between climatic elements and vegetation of rangelands in Hormozgan province
    (A Case Study Gymnocarpus decander). Natural Ecosystems of Iran, 6 (3):41-48. (In Persian)
  35. Kovalenko, P., Antonjuk, V., and Maliuta, S.  2004. Secondary metabolites synthesis in transformed cells of Glycyrrhiza glabra L. and Potentilla alba L. as producents of radioprotective compounds. Ukrainica Bioorganica Acta, 1(2): 13-22.
  36. Mukhopadhyay, M., and Panja, P. 2008. A novel process for extraction of natural sweetener from licorice (Glycyrrhiza glabra) roots. Separation Science and Technology, 63: 539–545.
  37. Parvaiz, M., Hussain, K., Khalid, S., Hussnain, N., and Iram, N.  2014. A Review: Medicinal Importance of Glycyrrhiza glabra L. (Fabaceae Family).  Global Journal of Pharmacology, 8: 8-13.
  38. Sanja, V., Filip, S., Izabella, S., Istvan, Z., Imre, O., and Suzana, J.S. 2018. Chemical composition, antioxidant and anticancer activity of licorice from Fruska Gora locality. Industrial Crops & Products, 112: 217–224.
  39. Saraf, B.D., Inam, F., and Deo, S.S. 2013. Antimicrobial and antioxidant activities of methanol extract roots of Glycyrrhiza glabra and HPLC analysis. International Journal of Pharmacy and Pharmaceutical Sciences, 5(2):157-160.
  40. Shabkhiz, M.A., Eikani, M.H., Golmohammad, F., and Bashiri Sadr, Z. 2016. Optimized Pressurized hot water extraction of glycyrrhizic acid from Licorice roots. Innovative Food Technologies, 2(4): 11-21. (In Persian)
  41. Siracusa, L., Saija, A., Cristani, M., Cimino, F., D’Arrigo, M., Trombetta, D., Rao, F., and  Ruberto, G. 2011. Phytocomplexes from liquorice (Glycyrrhiza glabra L.) leaves –chemical characterization and evaluation of their antioxidant, anti-genotoxic and anti-inflammatory activity. Fitoterapia, 82: 546–556.
  42. Slinkard, K. and Singleton, V.1977. Total phenolic analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28: 49-55.
  43. Sultana, S., Haque, A., Hamid, K., Urmi, K., and Roy, S.  2010. Antimicrobial, cytotoxic and antioxidant activity of methanolic extract of Glycyrrhiza glabra.  Agriculture and Biology Journal of North America, 1: 957–960.
  44. Szakiel, A., Pączkowski, C., and Henry, M., 2010. Influence of environmental abiotic factors on the content of saponins in plants. Phytochem. Rew, doi: 10.1007/s11101 -010-9177-x.
  45. Tawaha, K., Alali, F.Q., Gharaibeh, M., mohammad, M., and El-Elimat, T., 2007. antioxidant activity and total phenolic content of selected Jordanian plant species.  Food Chemistry, 104: 1372-8.
  46. Vaya, J., Belinky, P.A., and Aviram, M., 1997. Antioxidant constituents from licorice roots isolation, structure elucidation and antioxidative capacity toward LDL oxidation.  Free Radical Biology & Medicine, 23(2): 302-313.
  47. Wang, L., Yang, R., Yuan, B., Liu, Y., and Liu, C. 2015. The antiviral and antimicrobial activities of licorice, a widely-used Chinese herb.  Acta Pharmaceutica Sinica B, 5: 310–315.
  48. Yazdi, A., Sardari, S., Sayyaha, M., and Hassanpour Ezzati, M. 2011. Evaluation of the Anticonvulsant Activity of the Leaves of Glycyrrhiza glabra var. glandulifera Grown in Iran, as a Possible Renewable Source for Anticonvulsant Compounds. Iranian Journal of Pharmaceutical Research, 10 (1): 75-82.