بررسی اثر کادمیم بر تغییرات رنگدانه‌ای، فیتوشیمیایی و آنتی‌اکسیدانی سه گونه دارویی Mentha aquatica L.، Trautv. Eryngium caucasicum و Froriepia subpinnata Ledeb.

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه زراعت، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

2 گروه زراعت، دانشگاه علوم کشاورزی و منابع طبیعی ساری- ایران

3 ﮔﺮوه زراﻋﺖ و اﺻﻼح ﻧﺒﺎﺗﺎت، داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ واﺣﺪ ﻗﺎﺋﻢﺷﻬﺮ، ﻗﺎﺋﻢﺷﻬر، ایران

4 گروه خاک‌شناسی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری

چکیده

کادمیم یکی از خطرناکترین عناصر سنگین است که به طور طبیعی و یا با فعالیت‌های انسانی وارد خاک می‌شود و سبب تنش اکسیداتیو در گیاهان می‌شود. لذا این مطالعه، جهت بررسی اثر کادمیم بر برخی از واکنش‌های فیزیولوژیک سه گیاه معطر شامل پونه (Mentha aquatica L.)، زولنگ (Eryngium caucasicum Trautv.) و اناریجه(Froriepia subpinnata Ledeb.) ، انجام شد. در سه آزمایش گلخانه‌ای، پنج غلظت کادمیم خاک شامل 0، 5، 10، 15 و 20 میلی‌گرم کادمیم در کیلوگرم خاک، در قالب طرح کاملا تصادفی با چهار تکرار در دانشگاه علوم کشاورزی و منابع طبیعی ساری در سال 1396-1395 بررسی شد. نشاء هر سه گیاه در گلدان‌های آلوده کشت شدند. قبل از مرحله گلدهی کلروفیل a، b، کاروتنوئید، فنل، فلاونوئید و ظرفیت آنتی‌اکسیدان اندام‌های هوایی اندازه‌گیری شدند. نتایج نشان داد رنگدانه‌های برگ در هر سه گیاه با افزایش سطح کادمیم به صورت خطی کاهش یافت، اما میزان کاهش کاروتنوئید نسبت به کلروفیلa وb کمتر بود. همچنین کلروفیلb نسبت به کلروفیل a حساسیت بیشتری به کادمیم نشان داد. در حالی که فنل، فلاونوئیدها و ظرفیت آنتی‌اکسیدانی گیاهان با افزایش سطح کادمیم افزایش یافت. گیاه پونه بیشترین میانگین فنل (89/65 میلی‌گرم در گرم)، فلاونوئید (88/146 میلی‌گرم در گرم) و فعالیت آنتی‌اکسیدان (49/98 درصد) را به خود اختصاص داد. در مجموع نتایج نشان داد رنگدانه‌های گیاهی تحت تاثیر سمیت کادمیم قرار گرفتند و با توجه به این که گیاهان مورد نظر غنی از ترکیبات آنتی‌اکسیدان هستند؛ افزایش این ترکیبات در مواجه با تنش کادمیم نشان‌ دهنده‌ی نوعی مکانسیم دفاعی سه گیاه برای مقابله با تنش می-باشد.

کلیدواژه‌ها

موضوعات


References

  1. Amini, F., and Amirjani, M.R. 2013. Effect of Ni and Pb on chlorophyll content and metals accumulation in Medicago sativa. Journal of Crop Production and Processing, 2(6): 11-20.
  2. Anjum, N.A., Ahmad, I., Mohmood, I., Pacheco, M., Duarte, A.C., Pereira, E., Umar, S., Ahmad, A., Nafees A.K., Iqbal, M., and Prasad, M.N.V. 2012. Modulation of glutathione and its related enzymes in plants' responses to toxic metals and metalloids- a review. Environmental and Experimental Botany, 75: 307-324.
  3. Asgari Lajayer, H., Najafi, N., and Moghiseh, I. 2014. The effect of soil pollution to heavy metals on the production of medicinal plants. Journal of Land Management, 2(2): 111-122.
  4. Barandeh, F., and Kavousi, H.R. 2016. Effect of cadmium on changes of some enzymatic and none-enzymatic antioxidant defense systems in lentil seedlings (Lens culinaris Medik.). Iranian Journal of Pulses Research, 7(2): 125-137.
  5. Behera, R.K., and Mishra, P.C. 2002. High irradiance and water stress induce alterations in pigment composition and chloroplast activities of primary wheat leaves. Journal of Plant Physiology, 159: 967-973.
  6. Bonilla, J., Atarés, L., Chiralt, A., and Vargas, M. 2011. Recent patents on the use of antioxidant agents in food. Food, Nutrition and Agriculture, 3(2):123-132.
  7. Chang, C.C., Yang, M.H., Wen, H.M., and Chern, J.C. 2002. Estimation of total fla­vonoid content in vegetables by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10: 178-82.
  8. Demirci, B., Kosar, M., Demirci, F., Dinç, M., and Başer, K.H.C. 2007. Antimicrobial and antioxidant activities of the essential oil of Chaerophyllum libanoticum Boiss. Kotschy. Food Chemistry, 105(4): 1512-1517.
  9. Dias, M.C., Monteiro, C., Moutinho-Pereira, J., Correia, C., Goncalves, B., and Santos, C. 2013. Cadmium toxicity affects photosynthesis and plant growth at different levels. Acta Physiologiae Plantarum, 35(4): 1281-1289.
  10. Dudjak, J., Lachman, J., Miholova, D., Kolihova, D., and Pivec, V. 2004. Effect of cadmium on polyphenol content in young barley plants (Hordeum vulgare L.). Plant Soil Environment, 11(11): 471-477.
  11. Duruibe, J.O., Oguwuegbu, M.O.C., and Egwurugwu, J.N. 2007. Heavy metal pollution and human biotoxic effects. International Journal of Physical Sciences, 2(5): 112-118.
  12. Emmaline, J.K.J., Sharmila, S., and Jeyanthi L.R. 2016. Effect of heavy metals and UV irradiation on the production of flavonoids in Indigofera tinctoria. International Journal of Pharmaceutical Sciences Review and Research, 39(2): 104-107.
  13. Farhoosh, R., and Moosavi, S.M.R. 2006. Determination of carbonyl value in rancid oils: a critical reconsideration. Journal of Food Lipids, 13(3): 298-305.
  14. Firouzkoohi, F., Esmaeilzadeh Bahabadi, S., Mohkami, Z., and Yosefzaei, F. 2018. The effect of different solvents on total phenolic, flavonoid contents and antioxidant activity of different organs of Momordica charantia L. cultured in Sistan region. Eco-phytochemical Journal of Medicinal Plants, 5(4): 74-85.
  15. Ghorbani, H., Heidari, M., and Ghafari, M., 2016. Effect of salinity levels and lead and cadmium heavy metals on growth, photosynthetic pigments and sodium and potassium content in spinach. Journal of Science and Technology of Greenhouse Culture, 7(1): 15-24.
  16. Girdhar, M., Sharma, N.R., Rehman, H., Kumar, A., and Mohan, A. 2014. Comparative assessment for hyperaccumulatory and phytoremediation capability of three wild weeds. 3 Biotech, 4(6): 579-589.
  17. Good, A., and Zaplachiniski, S. 1994. The effects of drought on free amino acid accumulation and protein synthesis in Brassica napus. Physiolgia Plantarum, 90(1): 9-14.
  18. Habibzadeh, F., and Asghari, B. 2018. Study the effect of intercropping and chemical fertilizers on essential oil, phenolic and flavonoid contents and some biological properties of Hyssopus officinalis L. Eco-phytochemical Journal of Medicinal Plants, 3(6): 96-110.
  19. Hashem, A., Abd-Allah, E.F., Alqarawi, A.A., Malik, J.A., Wirth, S., and Egamberdieva, D. 2016. Role of calcium in AMF-mediated alleviation of the adverse impacts of cadmium stress in Bassia indica [Wight] A.J. Scott. Saudi Journal of Biological Sciences, 10: 1-11.
  20. Huang, Y., He, C., Shen, C., Guo, J., Mubeen, S., Yuan, J., and Yang, Z. 2017. Toxicity of cadmium and its health risks from leafy vegetable consumption. Food and Function, 8(4): 1373-1401.
  21. Jia, W., Lv, S., Feng, J., Li, J., Li, Y., and Li, S. 2016. Morphophysiological characteristic analysis demonstrated the potential of sweet sorghum (Sorghum bicolor (L.) Moench) in the phytoremediation of cadmium-contaminated soils. Environmental Science and Pollution Research, 23(18): 18823-18831.
  22. Kamal, M., Ghaly, A.E., Mahmoud, N., and Cote, R. 2004. Phytoaccumulation of heavy metals by aquatic plants. Environment International, 29(8): 1029- 1039.
  23. Khalid, N., Noman, A., Aqeel, M., Masood, A., and Tufail, A. 2018. Phytoremediation potential of Xanthium strumarium for heavy metals contaminated soils at roadsides. International Journal of Environmental Science and Technology, 15(97): 1-10.
  24. Khatib, M., Rashed Mohasel, M., Ganjali, A., and Lahouti, M. 2008. The effects of different nickel concentrations on some morpho-physiological characteristics of parsley (Petroselinum crispum). Iranian Journal of Field Crops Research, 2: 295-302.
  25. Kozminska, A., Wiszniewska, A., Hanus-Fajerska, E., and Muszynska, E. 2018. Recent strategies of increasing metal tolerance and phytoremediation potential using genetic transformation of plants. Plant Biotechnology Reports, 12(1): 1-14.
  26. Lavid, N., Schwartz, A., Yarden, O., and Tel-Or, E. 2001. The involvement of polyphenols and peroxides activities in heavy metal accumulation by epidermal glands of waterlily (Nymphaeceaea). Planta, 212(3): 323-331.
  27. Majer, B.J., Tscherko, D., and Paschke, A. 2002. Effects of heavy metal contamination of soils on micronucleus induction in Tradescantia and on microbial enzyme activities: a comparative investigation. Mutation Research, 515: 111-124.
  28. Manquián-Cerda, K., Escudey, M., Zuniga, G., Arancibia-Miranda, N., Molina, M., and Cruces, E. 2016. Effect of cadmium on phenolic compounds, antioxidant enzyme activity and oxidative stress in blueberry (Vaccinium corymbosum L.) plantlets grown in vitro. Ecotoxicology and Environmental Safety, 133: 316-326.
  29. Marquez-García, B., Fernández-Recamales, M.A., and Córdoba, F. 2012. Effects of cadmium on phenolic composition and antioxidant activities of Erica andevalensis. Journal of Botany, 1-6.
  30. Matsouka, I., Beri, D., Chinou, I., Haralampidis, K., and Spyropoulos, C.G. 2011. Metals and selenium induce medicarpin accumulation and excretion from the roots of fenugreek seedlings: a potential detoxification mechanism. Plant Soil Published online, 343(1-2): 235-245.
  31. Mauchamp, A., and Methy, M. 2004. Submergence-induced damage of photosynthetic apparatus in Phragmites australis. Environmental and Experimental Botany, 51(3): 227-235.
  32. Michalak, A. 2006. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies, 15(4): 523-530.
  33. Molazem, D., Qurbanov, E.M., and Dunyamaliyev, S.A. 2010. Role of proline, Na and chlorophyll content in salt tolerance of corn (Zea mays L.). American-Eurasian Journal of Agricultural and Environmental Science, 9(3): 319-324.
  34. Morteza-Semnani, K., Saeedi, M., and Akbarzadeh, M. 2009. The essential oil composition of Froriepia subpinnata (Ledeb.) Baill. Journal of Essential Oil Research, 21(2): 127-128.
  35. Mosavi, N., Ebadi, M., Khorshidi, M., Masoudian, N., and Hokmabadi H. 2018. Study of some physiological characteristics of potato tissue under salinity stress. International Journal of Farming and Allied Sciences, 7(1): 1-5.
  36. Nalousi, A., Hatamzadeh, A., Ghasemnezhad, M., and Alibiglouei, M. 2014. The study of physiological and biochemical responses of Agrostis stolonifera and Festuca arundinacea Schreb. Under drought stress. Iranian Journal of Plant Biology, 22: 105-116.
  37. Oh, M.M., Trick, H.N., and Rajasheka, C.B. 2009. Secondary metabolism and antioxidant are involved in environmental adaptation and stress tolerance in lettuce. Journal of Plant Physiology, 166(2): 180-191.
  38. Oliver, D., and Naidu, R. 2003. Uptake of Cu, Pb, Cd, As and DDT by vegetables grown in urban environments. National Environmental protection and Heritage council, Proceedings of the Fifth National Workshop on the Assessment of Site Contamination, 150-161.
  39. Parvaiz, A., and Satyawati, S. 2008. Salt stress and Phyto-biochemical responses of plants. Plant Soil Environment, 54: 89-99.
  40. Porra, R.J. 2002. The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. Photosynthesis Research, 73(1-3): 149-156.
  41. Prasad, S.M., and Singh, A. 2011. Metabolic responses of Azolla pinnatato cadmium stress: photosynthesis, antioxidative system and phytoremediation. Chemistry and Ecology, 27(6): 543-555.
  42. Ranjbar, M., Mohammadi, M. and Amjad, L. 2017. Lead and spermidine interact on physiological and biochemical indexes of plants Salvia officinalis L. Journal of Plant Process and Function Iranian Society of Plant Physiology, 6(21): 103-114.
  43. Rizwan, M., Ali, S., Adrees, M., Ibrahim, M., Tsang, D.C.W., Zia-ur-Rehman, M., Zahir, Z.A., Rinklebe, J., Tackf, F.M.G. and Ok, Y.S. 2017. A critical review on effects, tolerance mechanisms and management of cadmium in vegetables. Chemosphere, 182: 90-105.
  44. Saberi, M., Niknahad, H., Heshmati, G.A, Barani, H., and Alireza Shahriari, A.R. 2018. Evaluation of the content and performance of some active ingredients extracts of Citrullus colocynthis organs from two habitats of Sistan and Balochestan province in different growth stages. Journal of Plant Ecosystem Conservation, 6(11): 49-63.
  45. Sanchez-Moreno, C., Larrauri, J.A., and Saura-Calixto, F. 1999. Free radical scavenging capacity of selected red, rose and white wines. Journal of Agricultural and Food Chemistry, 79: 1301-1304.
  46. Shahid, M., Dumat, C., Khalid, S., Niazi, N.K., and Antunes, P.M.C. 2017. Cadmium bioavailability, uptake, toxicity and detoxification in soil-plant system. Reviews of Environmental Contamination and Toxicology, 241: 73-137.
  47. Sharma, P., and Dubey, R.S.H. 2005. Lead toxicity in plants. Brazilian Journal of Plant Physiology, 17(1): 35-52.
  48. Singleton, V.L., Orthofer, R., and Lamuela-Raventos, R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin Ciocalteu reagent. Methods in Enzymology, 299: 152-178.
  49. Tabatabaei, S., Shakeri, E., and Shahedi, M. 2013. Investigation of yield, yield components changes and some physiological characteristics of barley genotypes under irrigation tension conditions. Crop Physiology Journal, 5(18): 101-114.
  50. Wang, P., Su, Z., Yuan, W., Deng, G., and Li, S. 2012. Phytochemical constituents and pharmacological activities of Eryngium L. (Apiaceae). Pharmaceutical Crops, 3: 99-120.
  51. Xue, Z.C., Gao, H.Y., and Zhang, L.T. 2013. Effects of cadmium on growth, Photosynthetic rate, and chlorophyll content in leaves of soybean seedlings. Biologia Plantarum, 57(3): 587-590.
  52. Yaghoubian, Y., Siadat, S.A., Moradi Telavata, M.R., and Pirdashti, H. 2016. Quantify the response of purslane plant growth, photosynthesis pigments and photosystem II photochemistry to cadmium concentration gradients in the soil. Russian Journal of Plant Physiology, 63(1): 77-84.